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Green Dude
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Post subject: Cloning 101 Posted: Mon Jan 25, 2010 1:31 am |
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Joined: Thu Nov 12, 2009 1:40 am Posts: 907 Location: Approaching the southern cross
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Cloning is the process of making an exact copy of a living thing, in this case a marijuana plant. Cloning has many advantages to offer the advanced grower: first, the process of germination can be skipped over, saving valuable time. Second, the exact genetics of your best female can be preserved forever. Third, growers will save money by not having to purchase seeds every year. For SOG growers cloning is a must to ensure that only females will be grown and no space is wasted on males.
What do I need to start cloning? To begin cloning you will need a mother plant. A mother plant is the plant all of the cuttings will be taken from. It should exhibit qualities such as fast growth, high potency and mould and pest resistance. The mother should be kept in vegetative growth all the time under a MH light. Other things that will be needed are small plate), a shallow tray that will hold about three inches of water. An aquarium air pump, rooting hormone and high P fertilizer. Light will be provided by fluorescents hung seven inches above the plant tops.
Set-up First set the tray in a separate area from all the other plants. A separate closet or basement is good. Fill the tray with water (boiled for 20 minutes) mixed with liquid rooting hormone. Install the aquarium air pump to oxygenate the water. The size of the pump depends on the size of the tray. Make an anti-siphon loop in the hose to the pump to prevent water from getting into it if the power should go out. Next hang the fluorescents about 12 inches from the top of the tray. This will leave about 7 inches from the tops of the cuttings to the lights.
Taking the cuttings Once the cloning area is set up the clones can be taken from the mother plant. Taking clones is almost the same as pruning so when you prune instead of throwing away the cuttings they can be made into clones. The first cut should be made with sharp scissors or a razor blade sterilized with rubbing alcohol. The cutting should be about 6 to 8 inches tall. Next trim off the bottom leaves with a sharp razor blade. There should be three or four sets of leaves remaining on the cutting. Bring the cutting to the rooting tray and get out the Styrofoam plates. Poke a hole in the middle just big enough for the stem to fit through. The cutting can be secured in place with some sticky tack (looks like gum). Now submerge the bottom of the the cutting and make another cut diagonally. This will expose more stem surface to the water and make it easier for the cutting to absorb nutrients. This cut must be made under water to prevent a bubble forming in the stem, blocking nutrient intake.
Rooting the cuttings Now that the cuttings are floating in the rooting tray the water needs to be changed every other day. Just siphon it out and replace it quickly with new hormone treated water. Be careful never to drain all the water out. If the stem bottoms are exposed a bubble will developed inside the stem and the clone will die. After about 10 days in the rooting tray the stem bottom will start to come out slightly. This is the first sign that the roots will appear shortly. Foliar spray with a chemical fertilizer that is high in P at 1/4 strength every three days. Remember to spray the plants the day after foliar feeding with water to clean the leaves of any un-absorbed nutrients. Soon the tiny immature roots will be visible and when they reach about one inch in length they can be planted in the soil and will grow as an exact copy of the mother plant
Why must so many innocent ppl die , when we have a cure ?
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StonerBarbie
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Post subject: Re: Cloning 101 Posted: Tue Jan 26, 2010 3:33 am |
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Joined: Sat Jan 09, 2010 3:17 pm Posts: 1277 Location: The sky is green on my planet.
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wow, very nice. wish i had seen this when i took my first clone. ha
for entertainment purposes only. all information i present is false and untrue. just a bunch of pics i found on the internet.
viewtopic.php?f=11&t=1968
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Carolina_sinse
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Post subject: Re: Cloning 101 Posted: Tue Jan 26, 2010 5:22 am |
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Joined: Tue Dec 01, 2009 4:19 am Posts: 920 Location: Foothills of North Carolina
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The concept of clone does not mean that all members of the clone will necessarily appear identical in all characteristics. The phenotype that we observe in an individual is influenced by its surroundings. Therefore, members of the clone will develop differently under varying environmental conditions. These influences do not affect genotype and therefore are not permanent. Cloning theoretically can pre serve a genotype forever. Vigor may slowly decline due to poor selection of clone material or the constant pressure of disease or environmental stress, but this trend will re verse if the pressures are removed. Shifts in genetic composition occasionally occur during selection for vigorous growth. However, if parental strains are maintained by in frequent cloning this is less likely. Only mutation of a gene in a vegetative cell that then divides and passes on the mutated gene will permanently affect the genotype of the clone. If this mutated portion is cloned or reproduced sexually, the mutant genotype will be further replicated. Mutations in clones usually affect dominance relations and are therefore noticed immediately. Mutations may be induced artificially (but without much predictability) by treating meristematic regions with X-rays, colchicine, or other mutagens.
RESIDENTS OF NORTH CAROLINA SUPPORT HB1380 THE MEDICAL MARIJUANA ACT PLEASE CALL, E-MAIL YOUR REPS http://www.nccpn.org/index.php http://www.nccpn.org/forum/index.php
GET INVOLVED WITH MEDICAL CANNABIS ADVOCATES TO HELP US CHANGE LAWS
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Bak Alchisk-FMIF
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Post subject: Posted: Thu Feb 18, 2010 10:31 am |
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Joined: Sun Jan 10, 2010 8:04 pm Posts: 164 Location: ..in a lab coat and wearing glasses.
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We did an experiment with cloning in aero, which I will explain here for posterity.
We took 8 Hindu Kush clones, which were placed in a Daisy Cloner.
Each clone end was lightly scraped and dipped in RootTech cloning gel.
Four of the clones were then placed in the cloner bare-stemmed. Four were placed in Sure-2-Grow cubes.
The purpose of the experiment was to see that if clones placed in cubes would contain the gel for longer and root faster. To observe this, we cut the cubes with scissors all the way down one side. (The other purpose of cutting the cubes was so we could remove them once rooting had been established.)
Our results are that after 7 days, the clones in the cubes have approximately 1/2" roots showing well and healthy, while the clones without the cubes have root nubs on the stems, but no developed root systems yet.
Those clones with root systems will go into the vegetative unit today.
Peace, Bak Alchisk
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